nonlinear regression analysis using a one-phase decay model graphpad prism Search Results


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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
Graphpad Prism V6, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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one-phase exponential decay model in prism  (GraphPad Software Inc)
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using <t>one-phase</t> <t>exponential</t> <t>decay</t> <t>curve</t> <t>fitting</t> for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).
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(A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using one-phase exponential decay curve fitting for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).

Journal: Cell

Article Title: SWELL1, a plasma membrane protein, is an essential component of volume-regulated anion channel

doi: 10.1016/j.cell.2014.03.024

Figure Lengend Snippet: (A) Time course of relative cell volume change after the exposure of hypotonic solution (220 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 5). (B) RVD time constants were determined using one-phase exponential decay curve fitting for data shown in (A). Bars represent mean ± SEM (***p < 0.001). (C) Time course of the rate constant for swelling-induced [H3]taurine efflux after the exposure of hypotonic solution (210 mOsm/kg, indicated by the arrow) in HeLa cells transfected with either scrambled siRNA or siRNA against SWELL1. Each data point represents the mean ± SEM (n = 3).

Article Snippet: RVD time constants were determined using one-phase exponential decay curve fitting (GraphPad Prism 6).

Techniques: Transfection